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Ingo H. Stein, Verena Schüller, Philip Böhm, Prof. Dr. Philip Tinnefeld and Prof. Dr. Tim Liedl Single-Molecule FRET Ruler Based on Rigid DNA Origami Blocks ChemPhysChem 12

Version of Record online: 9 FEB 2011 | DOI: 10.1002/cphc.201000781

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FRET on a DNA origami structure: DNA origami assembly is used to position donor–acceptor pairs on the surface of DNA blocks for single-molecule energy transfer studies (see picture). In contrast to dsDNA FRET rulers, these origami blocks directly yield the expected distance dependence of energy transfer since the influence of the linkers on the donor–acceptor distance is reduced.

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