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Dr. Vicente L. Cebolla, Dr. Elena Mateos, Dr. Rosa Garriga, Dr. Carmen Jarne, Dr. Luis Membrado, Prof. Dr. Fernando P. Cossío, Dr. Eva M. Gálvez, Dr. Muriel Matt and Arantzazu Delgado-Camón Changes in Fluorescent Emission Due to Non-covalent Interactions as a General Detection Procedure for Thin-Layer Chromatography ChemPhysChem 13

Version of Record online: 3 NOV 2011 | DOI: 10.1002/cphc.201100590

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Fluorescence enhancement occurs when analytes bearing long hydrocarbon chains are added to ionic, dipolar or zwitterionic fluorophores impregnated on silica gel TLC plates due to non-covalent interactions between probe and target molecules. Hence the HPTLC peak height increases with increasing alkyl chain length, (see picture) alkanes. The effect of the increase in refractive index after analyte addition on the radiate emission,kr, is also investigated.

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