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Dr. Ouided Friaa, Melissa Furukawa, Aisha Shamas-Din, Dr. Brian Leber, Dr. David W. Andrews and Dr. Cécile Fradin Optimizing the Acquisition and Analysis of Confocal Images for Quantitative Single-Mobile-Particle Detection ChemPhysChem 14

Article first published online: 3 JUL 2013 | DOI: 10.1002/cphc.201201047

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Upwardly mobile: Diffusing particles appear as horizontal streaks in confocal images. The properties of the streaks are related to the fluorescence properties of the particles (mobility, specific brightness). A new theoretical framework is used to discuss optimal acquisition and analysis strategies of the confocal images, which allows a quantification of the single-mobile-particle properties within the concentration range 1–100 pM.

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