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Philipp Pinkerneil, Dr. Jörn Güldenhaupt, Prof. Dr. Klaus Gerwert and Dr. habil. Carsten Kötting Cover Picture: Surface-Attached Polyhistidine-Tag Proteins Characterized by FTIR Difference Spectroscopy (ChemPhysChem 11/2012) ChemPhysChem 13

Article first published online: 27 JUL 2012 | DOI: 10.1002/cphc.201290051

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ATR-FTIR spectroscopy monitors protein reactions and interactions of monolayers at the atomic level. On p. 2649 K. Gerwert, C. Kötting et al. describe a method to immobilize proteins by means of a polyhistidine-tag and nitrilotriacetic-acid-modified lipids. Because polyhistidine tags are often used for protein purification, modified proteins are easy to access. This makes the method a universal technique for many soluble proteins. The setup allows the investigation of the secondary structure, orientation, reaction mechanisms and protein–ligand or protein–protein interactions.

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