Won-Yong Jeon, Dr. Young-Bong Choi and Prof. Hyug-Han Kim Homogeneous Electrochemical Detection of Hippuric Acid in Urine Based on the Osmium–Antigen Conjugate ChemPhysChem 14
Metabolite measurement: Direct conjugation of an osmium complex to a small antigen (hippuric acid, HA) is applied to the quantitative analysis of the antigen and its antibody as the electrical signal of a screen-printed carbon electrode, for homogeneous competitive electrochemical immunoassay (see picture). The proposed immunoassay provides a simple, one-step, separation-free and inexpensive assay without washing steps. The electric signals increase linearly with the concentration of HA.
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