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Hung-Yi Wu and Prof. Dr. Hung-Wen Li Crowding Alters the Dynamics and the Length of RecA Nucleoprotein Filaments in RecA-Mediated Strand Exchange ChemPhysChem 15

Article first published online: 26 NOV 2013 | DOI: 10.1002/cphc.201300835

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Crowd impact: Molecular crowding effects of bovine serum albumin and poly(ethylene glycol) on the Escherichia coli RecA-mediated strand exchange reaction are quantified by using a single-molecule outgoing strand experiment and magnetic pull-down and ATPase assays. The alterations of the biochemical parameters of this complex enzymatic reaction in such crowded environments are discussed.

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