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Dr. Ryan J. Arant and Dr. Maximilian H. Ulbrich Deciphering the Subunit Composition of Multimeric Proteins by Counting Photobleaching Steps ChemPhysChem 15

Version of Record online: 31 JAN 2014 | DOI: 10.1002/cphc.201301092

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Photobleaching resolves protein structure! Determining the composition of macromolecules remains a basic question in biology. Until recently, measuring the protein stoichiometry in live cells has not been possible. This review focuses on the subunit counting technique, which takes advantage of photobleaching to count the number of subunits in a protein.

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