Yung-Han Shih, Sheng-Han Lo, Ni-Shin Yang, Brenda Singco, Yi-Jie Cheng, Cheng-You Wu, I-Hsin Chang, Prof. Hsi-Ya Huang and Prof. Chia-Her Lin Trypsin-Immobilized Metal–Organic Framework as a Biocatalyst In Proteomics Analysis ChemPlusChem 77
Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal–organic frameworks (MOFs). After separation by nano-LC-MS2 (liquid chromatography–mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.
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