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Hartmut M. Rapp, Stefanie Bacher, Anika Ahrens, Dr. Wolfgang Rapp, Dr. Bernd Kammerer, Prof. Dr. G. Ulrich Nienhaus and Prof. Dr. Willi Bannwarth Attachment of Proteins to Surfaces by Fluorous–Fluorous Interactions Restoring Their Structure and Activity ChemPlusChem 77

Version of Record online: 8 NOV 2012 | DOI: 10.1002/cplu.201200234

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Above the fold: An innovative strategy using a multifunctional linker system for the specific noncovalent immobilization of proteins to glass surfaces through fluorous–fluorous interactions without causing significant denaturation has been achieved. The principle was demonstrated for the enzymes RNase H and recombinant horseradish peroxidase (see figure).

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