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Lara Babich, Aloysius F. Hartog, Lieke J. C. van Hemert, Prof. Dr. Floris P. J. T. Rutjes and Prof. Dr. Ron Wever Synthesis of Carbohydrates in a Continuous Flow Reactor by Immobilized Phosphatase and Aldolase ChemSusChem 5

Article first published online: 13 NOV 2012 | DOI: 10.1002/cssc.201200468

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The dRAMA of arrested enzymes: A continuous flow reactor with three columns has been built with acid phosphatase and two different aldolases (RAMA and RhuA) immobilized on epoxy-functionalized beads (see figure). An enzymatic cascade reaction starting from dihydroxyacetone, pyrophosphate, and different aldehydes is performed in continuous mode to synthesize sugar analogues on a gram scale.

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