Sarah Al-Roubaie, Jasmine H. Hughes, Michael B. Filla, Rusty Lansford, Stephanie Lehoux and Elizabeth A.V. Jones Time-lapse microscopy of macrophages during embryonic vascular development Developmental Dynamics 241
Version of Record online: 30 JUL 2012 | DOI: 10.1002/dvdy.23835
We present a technique to double-label endothelial cells and macrophages with PKH26-PCL and AcLDL for in vivo time-lapsing.
The majority of double-labeled cells displayed an endothelial-like morphology, incorporating in the vessel wall and expressing Tie1, suggesting a myeloid-origin to some cells in the vessel wall.
Double-labeled cells that had integrated in the wall were largely immobile during normal development, however, could be recruited when remodeling was induced in the vascular network.
Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf
Required = Required Field