Sarah Al-Roubaie, Jasmine H. Hughes, Michael B. Filla, Rusty Lansford, Stephanie Lehoux and Elizabeth A.V. Jones Time-lapse microscopy of macrophages during embryonic vascular development Developmental Dynamics 241
Article first published online: 30 JUL 2012 | DOI: 10.1002/dvdy.23835
We present a technique to double-label endothelial cells and macrophages with PKH26-PCL and AcLDL for in vivo time-lapsing.
The majority of double-labeled cells displayed an endothelial-like morphology, incorporating in the vessel wall and expressing Tie1, suggesting a myeloid-origin to some cells in the vessel wall.
Double-labeled cells that had integrated in the wall were largely immobile during normal development, however, could be recruited when remodeling was induced in the vascular network.
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