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Sreelaja Nair, Robin E. Lindeman and Francisco Pelegri In vitro oocyte culture-based manipulation of zebrafish maternal genes Developmental Dynamics 242

Article first published online: 24 NOV 2012 | DOI: 10.1002/dvdy.23894

Key findings:

  • In vitro maturation method coupled to in vitro fertilization for the functional manipulation of maternal genes in zebrafish

  • Injection of wild-type mRNAs into oocytes rescues maternal-effect mutations

  • Functional knock down/reverse genetics can be achieved by expression of dominant-negative products or injection of translation-blocking morpholino oligonucleotides

  • Expression of mRNAs coding for fluorescent fusion products allows visualizing the subcellular localization of maternal factors immediately after fertilization

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