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James Spurlin III and Peter Lwigale A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations Developmental Dynamics 242

Version of Record online: 22 JAN 2013 | DOI: 10.1002/dvdy.23907

Key findings:

  • A multistep technique to dissect extraembryonic membranes away from the developing chick embryo.
  • This technique permits access to late-stage chick embryos (between E5 and E8) in ovo, which is otherwise difficult, as the extraembryonic membranes obscure chick embryos shortly after E4.
  • Exposed late-stage chick embryos are viable and can be manipulated using standard developmental biology techniques to study organogenesis.

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