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Juan Fernández and Ricardo Fuentes Fixation/Permeabilization: New Alternative Procedure for Immunofluorescence and mRNA In Situ Hybridization of Vertebrate and Invertebrate Embryos Developmental Dynamics 242

Version of Record online: 15 MAR 2013 | DOI: 10.1002/dvdy.23943

Key Findings:

  • Our fixation/permeabilization procedure for immunofluorescence and mRNA in situ hybridization is a highly versatile procedure that works efficiently in diverse cell types of embryos of different taxa at different developmental stages.
  • The same fixation/permeabilization procedure allows independent or simultaneous immunofluorescence staining and mRNA in situ hybridization.
  • The fixation/permeabilization procedure combines good structural preservation of the cytoplasm with high reactivity of immunogenic sites and mRNA.
  • Fixation/permeabilization allows good immunofluorescence staining and mRNA in situ hybridization of both whole-mounted and cryosectioned embryos.
  • Short C-chain carboxylic acids appear to modulate the rate at which formaldehyde cross-links cell proteins.

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