E-mail

E-mail a Wiley Online Library Link

Meng-Chieh Shen, A. Tuba Ozacar, Marcey Osgood, Crina Boeras, Jochebed Pink, Jeanne Thomas, Jhumku D. Kohtz and Rolf Karlstrom Heat-shock–mediated conditional regulation of hedgehog/gli signaling in zebrafish Developmental Dynamics 242

Version of Record online: 27 MAR 2013 | DOI: 10.1002/dvdy.23955

Key Findings:

  • Four new heat-shock–inducible transgenic lines allow temporal manipulation of Hedgehog (Hh) signaling in zebrafish.
  • Heat shock of these lines effectively activates or represses Hh signaling through embryonic and early larval stages. Hh signaling is disrupted, but to a lesser degree, at larval and early adult stages.
  • Hh signaling can be cell-autonomously increased or decreased at the transcriptional level, or activated non–cell-autonomously at the level of the Sonic Hh (Shh) ligand.
  • Two new Hh/Gli reporter lines sensitively label Hh responding cells throughout the life cycle and allow visualization of graded Hh responses in the CNS.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field

SEARCH