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Meng-Chieh Shen, A. Tuba Ozacar, Marcey Osgood, Crina Boeras, Jochebed Pink, Jeanne Thomas, Jhumku D. Kohtz and Rolf Karlstrom Heat-shock–mediated conditional regulation of hedgehog/gli signaling in zebrafish Developmental Dynamics 242

Version of Record online: 27 MAR 2013 | DOI: 10.1002/dvdy.23955

Key Findings:

  • Four new heat-shock–inducible transgenic lines allow temporal manipulation of Hedgehog (Hh) signaling in zebrafish.
  • Heat shock of these lines effectively activates or represses Hh signaling through embryonic and early larval stages. Hh signaling is disrupted, but to a lesser degree, at larval and early adult stages.
  • Hh signaling can be cell-autonomously increased or decreased at the transcriptional level, or activated non–cell-autonomously at the level of the Sonic Hh (Shh) ligand.
  • Two new Hh/Gli reporter lines sensitively label Hh responding cells throughout the life cycle and allow visualization of graded Hh responses in the CNS.

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