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Cécile Tron, Mickaël V. Cherrier, Patricia Amara, Lydie Martin, Francois Fauth, Edmundo Fraga, Mélanie Correard, Marc Fontecave, Yvain Nicolet and Juan C. Fontecilla-Camps Further Characterization of the [FeFe]-Hydrogenase Maturase HydG European Journal of Inorganic Chemistry 2011

Article first published online: 2 FEB 2011 | DOI: 10.1002/ejic.201001101

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We have explored the CO/CN synthesis catalyzed by [FeFe]-hydrogenase maturase HydG with site-directed mutants, and have performed functional studies, SAXS spectroscopy and homology modeling. We conclude that whereas CN is made in the active site-containing TIM-barrel domain, the CO precursor follows an internal path from this site to a catalytic [4Fe-4S] cluster in the tightly bound C-terminal domain.

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