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Yoshinao Katsu, Anke Lange, Shinichi Miyagawa, Hiroshi Urushitani, Norishisa Tatarazako, Yukio Kawashima, Charles R. Tyler and Taisen Iguchi Cloning, expression and functional characterization of carp, Cyprinus carpio, estrogen receptors and their differential activations by estrogens Journal of Applied Toxicology 33

Version of Record online: 1 JUL 2011 | DOI: 10.1002/jat.1707

Environmental estrogens can influence the reproductive system and have been shown to disrupt gametogenesis in males. We cloned all three carp Cyprinus carpio, estrogen receptors [ER; ERa, ERb1 and ERb2] and applied a transient transfection ERE-luciferase reporter assay system using mammalian cells. cERb2 showed a higher sensitivity to the natural steroid oestrogen, 17b-estradiol, than cERa. This is a powerful assay for toxicology, and provides a tool for future studies examining the receptor-environmental chemical interactions and estrogen disrupting mechanisms in carp.

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