Mingming Hu, Lina Xu, Lianhong Yin, Yan Qi, Hua Li, Youwei Xu, Xu Han, Jinyong Peng and Xianyao Wan Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways Journal of Applied Toxicology 33
In this study, Table 1 shows the information of primer, anneal temperature and cycle of RT-PCR. PCR of all genes was performed under the conditions in this table. β-Actin was chosen as house-keeping gene. Table 2 is the results of apoptosis assay by flow cytometry. In the presence of dioscin, the rate of early apoptosis, advanced stage apoptosis and necrosis of cancer cells was increased in a dose-dependent manner.
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