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Purificación Fernández, Santiago Seoane, Cristina Vázquez, María Jesús Tabernero, Antonia M. Carro and Rosa A. Lorenzo Chromatographic determination of drugs of abuse in vitreous humor using solid-phase extraction Journal of Applied Toxicology 33

Version of Record online: 15 FEB 2012 | DOI: 10.1002/jat.2722

A method is presented for the determination of drugs of abuse in vitreous humor using high-performance liquid chromatography and solid-phase extraction. A linear response from the detector was obtained within the concentration range of 0.1–4 µg mL−1, the limits of detection were lower than 30 ng mL−1, the coefficients of variation fluctuated between 0.1% and 12.4%, and the average recoveries were >78% for all the drugs except for EDDP, with a value of 66.4%.

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