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María José Polanco, Luis Fernando Alguacil and Carmen González-Martín Pro-apoptotic properties of morphine in neuroblastoma × glioma NG108-15 hybrid cells: modulation by yohimbine Journal of Applied Toxicology 34

Version of Record online: 12 SEP 2012 | DOI: 10.1002/jat.2817

Morphine neurotoxicity has been studied in NG108-15 cells after 24 h incubation with 0.1 and 10 μM concentrations of the opioid. Images compatible with apoptosis and necrosis could be observed directly and after staining with methylene blue, crystal violet and propidium iodide/4′,6-diamidino-2-phenylindole. Quantification of caspase 3/7 activity and DNA fragmentation confirmed a modest but significant proapoptotic effect of morphine. Co-incubation with 10 μM yohimbine prevented all these changes, thus extending previous findings of a yohimbine-sensitive, neurotoxic effect of morphine on NG108-15 cells.

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