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Ikumi Hirakawa, Shinichi Miyagawa, Naoko Mitsui, Maki Miyahara, Yuta Onishi, Yoshihiro Kagami, Teruhiko Kusano, Takashi Takeuchi, Yasuhiko Ohta and Taisen Iguchi Developmental disorders and altered gene expression in the tropical clawed frog (Silurana tropicalis) exposed to 17α-ethinylestradiol Journal of Applied Toxicology 33

Version of Record online: 6 NOV 2012 | DOI: 10.1002/jat.2836

The occurrence of oocytes in the testis (testis-ova) is one reproductive disorder and can be used as a valid endpoint when studying disruptive effects of estrogenic chemicals in fish and amphibians. Gonads of the genetically male tropical clawed frog (Silurana tropicalis) exposed developmentally to 17α-ethinylestradiol were analyzed using histopathology, microarray and quantitative PCR. Expression of zpc and 42Sp50 genes was demonstrated to be associated with testis-ova in the S. tropicalis.

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