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Yin-Hua Xiong, Ying Xu, Li Yang and Zheng-Tao Wang Gas chromatography–mass spectrometry-based profiling of serum fatty acids in acetaminophen-induced liver injured rats Journal of Applied Toxicology 34

Version of Record online: 12 DEC 2012 | DOI: 10.1002/jat.2844

A simple, accurate and sensitive gas chromatography-mass spectrometry (GC-MS) method has been developed and validated for analyzing the metabolic profile of fatty acids and simultaneous quantification of 18 fatty acids including non-esterified fatty acids (NEFA) and esterified fatty acids (EFA) in acetaminophen (APAP)-induced liver injured and control rats serum.

Along with the chemometrics analyses of principle component analysis (PCA) and partial least squares- discriminant analysis (PLS-DA), APAP-induced and control rats could be differentiated and NEFAs such as oleic acid (C18:1n9), linoleic acid (C18:2n6), docosahexaenoic acid (C22:6n3) and arachidonic acid (C20:4n6) were identified as the potential biomarkers of APAP-induced hepatic injury.

The novel combined study of GC-MS analysis and chemometric analysis based on the metabolic profile of fatty acids was a prospective technique for the assessment of chemical-induced hepatotoxicity.

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