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Jessica Rach, Jessica Budde, Niklas Möhle and Michaela Aufderheide Direct exposure at the air–liquid interface: evaluation of an in vitro approach for simulating inhalation of airborne substances Journal of Applied Toxicology 34

Article first published online: 14 JUN 2013 | DOI: 10.1002/jat.2899

A novel technique for exposure of cell cultures at the air–liquid interface (ALI) to airborne materials is introduced. The efficiency of the experimental setup with the CULTEX® Radial Flow System (RFS) is demonstrated by dose-response investigations of cigarette smoke and airborne particles. The value of such in vitro approaches as an alternative to current animal experiments for inhalation toxicology is discussed.

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