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Rachel P. L. van Swelm, Mackenzie Hadi, Coby M. M. Laarakkers, Rosalinde Masereeuw, Geny M. M. Groothuis and Frans G. M. Russel Proteomic profiling in incubation medium of mouse, rat and human precision-cut liver slices for biomarker detection regarding acute drug-induced liver injury Journal of Applied Toxicology 34

Article first published online: 30 AUG 2013 | DOI: 10.1002/jat.2917

Drug-induced liver injury often leads to drug withdrawal. Proteins released from human, mouse and rat precision-cut liver slices (PCLS) exposed to several hepatotoxicants were profiled using MALDI-TOF MS. Following acetaminophen treatment, similar changes in protein profiles (including the key proteins SOD1, CA3 and calmodulin) were demonstrated as previously found in vivo in mouse urine. Incubation with diclofenac resulted in the same toxicity parameters as observed for acetaminophen. In conclusion, proteomics can be applied to identify potential translational biomarkers in PCLS.

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