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Sebastian Klein, Daniel Mueller, Valery Schevchenko and Fozia Noor Long-term maintenance of HepaRG cells in serum-free conditions and application in a repeated dose study Journal of Applied Toxicology 34

Version of Record online: 30 SEP 2013 | DOI: 10.1002/jat.2929

HepaRG cells were maintained viable with constant CYP activities and liver-specific functions for 30 days in serum-free medium (SFM). The long-term viability and CYP activities were superior to those reported for primary human hepatocytes in vitro. Metabolic flux analysis indicates that the central carbon metabolism in SFM is comparable to standard conditions. This system can be applied for repeated dose toxicity assessments and will pave way for application on high content ‘omics’ platform in systems toxicology.

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