Deanna Wolfson, Michael Steck, Martin Persson, Gregory McNerney, Ana Popovich, Mattias Goksör and Thomas Huser Rapid 3D fluorescence imaging of individual optically trapped living immune cells Journal of Biophotonics 8
An approach is demonstrated to rapidly characterize living suspension cells in 4 dimensions while they are immobilized and manipulated within optical traps. A single, high numerical aperture objective lens is used to separate the imaging plane from the trapping plane. This facilitates full control over position and orientation of multiple trapped cells using a spatial light modulator, including directed motion and object rotation, while also allowing rapid 4D imaging.
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