Xinxin Zhu, Luyuan Zhang, Ya-Ting Kao, Fang Xu and Wei Min A tunable fluorescent timer method for imaging spatial-temporal protein dynamics using light-driven photoconvertible protein Journal of Biophotonics 8
A light-driven timer concept is proposed that could report relative protein ages at specific sub-cellular locations, by weakly but chronically illuminating photoconvertible fluorescent proteins inside cells. This method exploits light, instead of oxygen, as the driving force. Therefore its timing speed is optically tunable by adjusting the photoconverting laser intensity. This timer method offers a flexible "ruler" for studying temporal hierarchy of spatially ordered processes with exquisite spatial-temporal resolution.
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