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Thorsten Kolb, Sahradha Albert, Michael Haug and Graeme Whyte Optofluidic rotation of living cells for single-cell tomography Journal of Biophotonics 8

Version of Record online: 14 APR 2014 | DOI: 10.1002/jbio.201300196

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Flow cytometry provides a high throughput, multi-dimensional analysis of cells flowing in suspension. In order to combine this feature with the ability to resolve detailed structures in 3D, an optofluidic device was developed that combines a microfluidic system with a dual beam trap. The combination of both techniques enables the tomographic reconstruction of the 3D structure of the cell. In addition this method is capable to provide detailed 3D structural data for flow cytometry.

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