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Jonas Mücksch, Thiemo Spielmann, Evangelos Sisamakis and Jerker Widengren Transient state imaging of live cells using single plane illumination and arbitrary duty cycle excitation pulse trains Journal of Biophotonics 8

Version of Record online: 7 APR 2014 | DOI: 10.1002/jbio.201400015

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The applicability of Single Plane Illumination Microscopy to Transient State Imaging (TRAST), offering sensitive microenvironmental information together with optical sectioning and reduced overall excitation light exposure of the specimen is demonstrated. The concept is verified by showing that transition rates can be determined accurately for free dye in solution and that fluorophore transition rates can be resolved pixel-wise in live cells. Furthermore, a new theoretical framework for analyzing TRAST data acquired with arbitrary duty cycle pulse trains is derived.

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