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Christian Brackmann, Anton Bengtsson, Marie Larsson Alminger, Ulf Svanberg and Annika Enejder Visualization of β-carotene and starch granules in plant cells using CARS and SHG microscopy Journal of Raman Spectroscopy 42

Version of Record online: 28 AUG 2010 | DOI: 10.1002/jrs.2778

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Non-linear microscopy is introduced for three-dimensional, label-free imaging of provitamin-A-carotenoids and starch granules in plant tissues. Carotenoids in orange-fleshed sweet potato (OFSP), carrot and mango were visualized by CARS microscopy and related to the plant-matrix morphology by simultaneous SHG microscopy of starch granules. While OFSP and carrot showed rod-shaped bodies with high carotenoid densities, the carotenoid-filled lipid droplets in mango appeared as low-density aggregates of rounded shape. Thermal processing of OFSP showed intact carotenoid bodies despite significant changes of the surrounding starch granules. This illustrates the value of non-linear microscopy also within the plant biology and food sciences.

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