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Stanislav O. Konorov, H. Georg Schulze, James M. Piret, Robin F. B. Turner and Michael W. Blades Evidence of marked glycogen variations in the characteristic Raman signatures of human embryonic stem cells Journal of Raman Spectroscopy 42

Article first published online: 23 NOV 2010 | DOI: 10.1002/jrs.2829

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Human embryonic stem cells (hESCs) have distinctive Raman signatures that are potentially useful for nondestructively distinguishing these pluripotent cells from differentiating cells. These signatures may vary during routine hESC culture maintenance and could also be affected by subculturing. In measured spectra, glycogen was identified as the major contributor to spectral variations. This was further confirmed by the similarity between a Raman map of glycogen distribution and a periodic acid Schiff (PAS, carbohydrates) stain of the same area.

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