Juqiang Lin, Rong Chen, Shangyuan Feng, Jianji Pan, Buhong Li, Guannan Chen, Shaojun Lin, Chao Li, Li-qing Sun, Zufang Huang and Haishan Zeng Surface-enhanced Raman scattering spectroscopy for potential noninvasive nasopharyngeal cancer detection Journal of Raman Spectroscopy 43
Combining membrane electrophoresis with surface-enhanced Raman scattering (SERS) spectroscopy, serum proteins were first purified and then mixed with silver nanoparticles to perform SERS spectral analysis. Therefore, the spectral signatures were enhanced to high-fidelity SERS signatures because of the purification procedure of the first step. We used the method to analyze blood plasma samples from nasopharyngeal cancer patients (n=43) and healthy volunteers (n=33) for cancer detection. Principal components analysis of the SERS spectra revealed that the data points for the cancer groups and the normal group form distinct, completely separated clusters with no overlap. Therefore, the nasopharyngeal cancer group can be unambiguously discriminated from the normal group, i.e., with both diagnostic sensitivity and specificity of 100%. These results are very promising for developing a label-free, noninvasive, and reliable clinical tool for rapid cancer detection and screening.
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