Alessandro Virga, Paola Rivolo, Emiliano Descrovi, Alessandro Chiolerio, Gabriella Digregorio, Francesca Frascella, Marco Soster, Federico Bussolino, Serena Marchiò, Francesco Geobaldo and Fabrizio Giorgis SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide–antibody assays Journal of Raman Spectroscopy 43
Version of Record online: 24 NOV 2011 | DOI: 10.1002/jrs.3086
Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface-enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.
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