S. Mangialardo, F. Piccirilli, A. Perucchi, P. Dore and P. Postorino Raman analysis of insulin denaturation induced by high-pressure and thermal treatments Journal of Raman Spectroscopy 43
Raman spectroscopy has been used to investigate different conformational states of insulin: the native form and several structurally modified states with different extent of denaturation induced by thermo-chemical treatment and by applying very high pressure (up to 8 GPa) using a diamond anvil cell. High-pressure results confirm the peculiar strength to volume compression of insulin and largely extend the pressure range of its structural stability. Above 4.2 Gpa, insulin undergoes an irreversible transition to a new conformational state.
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