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Carolin Blum, Thomas Schmid, Lothar Opilik, Simon Weidmann, Stephan R. Fagerer and Renato Zenobi Understanding tip-enhanced Raman spectra of biological molecules: a combined Raman, SERS and TERS study Journal of Raman Spectroscopy 43

Version of Record online: 22 JUN 2012 | DOI: 10.1002/jrs.4099

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Confocal Raman, surface-enhanced Raman and tip-enhanced Raman spectra of peptides and a protein are compared to learn from their differences and similarities. We show that (1) the widely used assumption that spectral bands do not shift when comparing these methods is valid, (2) the band intensities can vary dramatically and (3) marker bands can be assigned, which should allow one to localize proteins in a complex environment. General guidelines for the interpretation of tip-enhanced Raman spectra of biological molecules are presented.

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