E-mail a Wiley Online Library Link

Aijing Wang, Weidong Ruan, Wei Song, Lei Chen, Bing Zhao, Young Mee Jung and Xu Wang Detection of the potential tumor marker of AFP using surface-enhanced Raman scattering-based immunoassay Journal of Raman Spectroscopy 44

Article first published online: 30 SEP 2013 | DOI: 10.1002/jrs.4391

Thumbnail image of graphical abstract

In this paper, we propose a universal, rapid, sensitive, and highly specific immunoassay system utilizing gold nanoparticles (AuNPs) and surface-enhanced Raman scattering (SERS). This new system features a sandwich structure combining mercaptobenzoic acid-labeled immunogold nanoparticles with the antigen and the antibody atop a pre-designed substrate made of a glass slide modified with AuNPs. This SERS-based immunoassay can detect AFP concentrations as low as 100 pg/ml, which is a significant improvement on the capabilities of the enzyme-linked immunosorbent assay method. This technique provides an effective model for the detection of biomarkers in medical diagnostics, criminal investigation, and other fields.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field