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Piotr J. Mak, Qianhong Zhu and James R. Kincaid Using resonance Raman cross-section data to estimate the spin-state populations of cytochromes P450 Journal of Raman Spectroscopy 44

Version of Record online: 11 OCT 2013 | DOI: 10.1002/jrs.4401

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The cytochromes P450 are monooxygenases important in the elimination of xenobiotics and in the biosynthesis of essential steroid products. Substrate binding initiates the enzymatic cycle, converting ferric low-spin to high-spin state. Here, the methodology is developed to permit resonance Raman spectroscopy to be effective in gaining this information. This method is shown to permit a reliable calculation of relative populations of the two spin states from spectra of several other cytochromes P450, an indication of its promise for general usage.

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