Piotr J. Mak, Qianhong Zhu and James R. Kincaid Using resonance Raman cross-section data to estimate the spin-state populations of cytochromes P450 Journal of Raman Spectroscopy 44
The cytochromes P450 are monooxygenases important in the elimination of xenobiotics and in the biosynthesis of essential steroid products. Substrate binding initiates the enzymatic cycle, converting ferric low-spin to high-spin state. Here, the methodology is developed to permit resonance Raman spectroscopy to be effective in gaining this information. This method is shown to permit a reliable calculation of relative populations of the two spin states from spectra of several other cytochromes P450, an indication of its promise for general usage.
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