Mariana Tasso, Ana L. Cordeiro, Katrin Salchert and Carsten Werner Covalent Immobilization of Subtilisin A onto Thin Films of Maleic Anhydride Copolymers Macromolecular Bioscience 9
We present a versatile strategy for the immobilization of Subtilisin A to surfaces using different types of maleic anhydride copolymer coatings for the covalent anchorage of the enzyme. The performance of the immobilized enzyme was shown to be influenced by the physicochemical characteristics of the polymer support. Higher amounts and activities of the protease were obtained on hydrophilic, swollen (PEMA) coatings.
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