Kayla A. Kaiser, Gregory A. Barding Jr and Cynthia K. Larive A comparison of metabolite extraction strategies for 1H-NMR-based metabolic profiling using mature leaf tissue from the model plant Arabidopsis thaliana Magnetic Resonance in Chemistry 47
This work compares several commonly used metabolite extraction solvents for their effects on the 1H-NMR metabolic profile of the model plant Arabidopsis thaliana. Perchloric acid and D2O extracts showed evidence of sucrose degradation. Mixed organic-aqueous extraction solvents had better stability but gave rise to problems due to contamination from lipid resonances. The best results were obtained performing the extraction with CD3CN/D2O followed by removal of solvent and reconstitution in D2O buffer.
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