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Kayla A. Kaiser, Gregory A. Barding Jr and Cynthia K. Larive A comparison of metabolite extraction strategies for 1H-NMR-based metabolic profiling using mature leaf tissue from the model plant Arabidopsis thaliana Magnetic Resonance in Chemistry 47

Article first published online: 23 JUN 2009 | DOI: 10.1002/mrc.2457

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This work compares several commonly used metabolite extraction solvents for their effects on the 1H-NMR metabolic profile of the model plant Arabidopsis thaliana. Perchloric acid and D2O extracts showed evidence of sucrose degradation. Mixed organic-aqueous extraction solvents had better stability but gave rise to problems due to contamination from lipid resonances. The best results were obtained performing the extraction with CD3CN/D2O followed by removal of solvent and reconstitution in D2O buffer.

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