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Ahmed Emad and Régen Drouin Evaluation of the impact of density gradient centrifugation on fetal cell loss during enrichment from maternal peripheral blood Prenatal Diagnosis 34

Version of Record online: 1 JUN 2014 | DOI: 10.1002/pd.4387

What's already known about this topic?

  • We recently developed a robust algorithm for detection of rare cellular events using fluorescence-based automated microscopy and used the sequential spreading slide approach in the comparison of manual and automatic scanning of slides with predefined number and location of rare male cells in a pure population of female cells. Consequently, we tested this classifier for the detection of fetal cells from maternal blood of both normal and aneuploid pregnancies with Down syndrome. We validated the direct detection strategy of fetal cells and offered conclusive data of the absolute number of these cells per milliliter of maternal blood without prior enrichment by automated microscopy.

What does this study add?

  • In this study, we applied this methodology in the evaluation of fetal cell loss during enrichment by density gradient centrifugation and explored its etiology. We also presented a modified way of density gradient, which reduces fetal cell loss to be considered in perspective enrichment protocols.

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