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Sudarsan Vishnupriya, Kamalesh Chaudhari, Ramya Jagannathan and Thalappil Pradeep Single-Cell Investigations of Silver Nanoparticle–Bacteria Interactions Particle & Particle Systems Characterization 30

Article first published online: 1 JUL 2013 | DOI: 10.1002/ppsc.201300165

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The interactions of E. coli with citrate-reduced silver nanoparticles (AgNPs) are studied using Raman spectroscopy and plasmon resonance imaging of single bacterial cells. While attachment of nanoparticles to bacteria is evident in plasmonic hyperspectral imaging, molecular changes of the adsorbed DNA are seen in Raman spectra. Specific interactions of AgNPs with exocyclic nitrogen present in the bases, adenine, guanine, and cytosine leads to time-dependent changes in the DNA.

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