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Ahmad Asoodeh, Hadi Zare Zardini and JamshidKhan Chamani Identification and characterization of two novel antimicrobial peptides, temporin-Ra and temporin-Rb, from skin secretions of the marsh frog (Rana ridibunda) Journal of Peptide Science 18

Article first published online: 29 SEP 2011 | DOI: 10.1002/psc.1409

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Two novel antimicrobial peptides from the skin secretions of Rana ridibunda, named temporin-Ra and temporin-Rb, were purified and characterized. These peptides have inhibitory effects on both gram-negative, gram-positive bacteria and two antibiotic-resistant strains prevalent in hospitals. Minimum inhibition concentration (MIC) values of these peptides are suitable for potent antimicrobial peptides. The peptides do not have hemolytic effect against human red blood cells (RBC) in their MIC values. These peptides showed 1.3% hemolysis at a concentration of 60 µg/ml.

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