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Reena Gyanda, Jayati Banerjee, Yi-Pin Chang, Angela M. Phillips, Lawrence Toll and Christopher J. Armishaw Oxidative folding and preparation of α-conotoxins for use in high-throughput structure–activity relationship studies Journal of Peptide Science 19

Version of Record online: 28 NOV 2012 | DOI: 10.1002/psc.2467

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The effect of co-solvent, redox reagents, pH, temperature, and time were systematically investigated for α-conotoxins MII, PnIB, GID, AuIB, BuIA, ImI, and GI to determine the optimized conditions for folding to the native disulfide bond isomer. We have also demonstrated an efficient ‘semi-purification’ technique for the high-throughput isolation of α-conotoxins following oxidative folding. Our simplified procedures permit the rapid identification of active hits directly from high-throughput pharmacological screening assays.

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