Yosuke Suzuki, Hiroki Itoh, Fumihiko Katagiri, Fuminori Sato, Yukie Sato, Kanako Kawasaki, Yuhki Sato, Hiromitsu Mimata and Masaharu Takeyama Development and clinical application of an enzyme immunoassay for the determination of midregional proadrenomedullin Journal of Peptide Science 19
In this study, we attempted to develop an enzyme immunoassay (EIA) for quantifying mid-regional proadrenomedullin (MR-proADM)-like immunoreactive substance (IS), which is applicable for monitoring plasma MR-proADM levels. Using β-D-galactosidase-labeled preproADM(83-94) as a marker antigen, anti-rabbit IgG-coated immunoplate as a bound/free separator, and 4-methylumbelliferyl-β-D-galactopyranoside as a fluorogenic substrate, a sensitive and specific EIA was developed for the quantification of MR-proADM-IS in human plasma. Our EIA may be useful to evaluate plasma MR-proADM levels as a biomarker in various clinical settings.
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