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Orestis Faklaris, Damien Garrot, Vandana Joshi, Frédéric Druon, Jean-Paul Boudou, Thierry Sauvage, Patrick Georges, Patrick A. Curmi and François Treussart Detection of Single Photoluminescent Diamond Nanoparticles in Cells and Study of the Internalization Pathway Small 4

Version of Record online: 6 NOV 2008 | DOI: 10.1002/smll.200800655

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Diamond nanoparticles are promising photoluminescent probes for tracking intracellular processes, due to embedded, perfectly photostable color centers. The spontaneous internalization process of such nanoparticles (diameter 25 nm) in HeLa cancer cells is investigated by confocal microscopy and time-resolved techniques. The preliminary conclusion is that nanodiamonds are not trapped in endosomes (low colocalisation fraction, marked by yellow arrow on the figure).

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