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Thomas Crouzier, Kefeng Ren, Claire Nicolas, Christian Roy and Catherine Picart Layer-By-Layer Films as a Biomimetic Reservoir for rhBMP-2 Delivery: Controlled Differentiation of Myoblasts to Osteoblasts Small 5

Article first published online: 13 FEB 2009 | DOI: 10.1002/smll.200800804

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Kept in reserve: Controlled differentiation of myoblast cells in myotubes (see images, top) or osteoblasts (bottom) occurs via poly(L-lysine)/hyaluronan (PLL/HA) multilayer films loaded with increasing amounts of recombinant human bone morphogenetic protein 2 (rhBMP-2). Myogenic (troponin T, green) and osteogenic (alkaline phosphatase, blue) markers are used.

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