Jung-Reem Woo, Dong-Kwon Lim and Jwa-Min Nam Minimally Stable Nanoparticle-Based Colorimetric Assay for Simple, Rapid, and Sensitive Antibody Structure and Activity Evaluation Small 7
A simple and rapid assay is developed that allows for the determination of antibody structure and activity using minimally stable gold nanoparticle (AuNP) probes. The addition of salt to a minimally stable particle solution expedites particle aggregation and a subsequent color change in the solution. This colorimetric assay is highly sensitive and the whole assay time is ≈25 min without any complicated instrumentation.
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