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Yu Pan, Annika Leifert, Michael Graf, Frank Schiefer, Sven Thoröe-Boveleth, Janine Broda, Mary C. Halloran, Henner Hollert, Dominic Laaf, Ulrich Simon and Willi Jahnen-Dechent High-Sensitivity Real-Time Analysis of Nanoparticle Toxicity in Green Fluorescent Protein-Expressing Zebrafish Small 9

Article first published online: 12 NOV 2012 | DOI: 10.1002/smll.201201173

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Ultrasmall triphenylphosphine-capped gold nanoparticles (AuNP) continuously generate reactive oxygen species (ROS), causing cell stress. This triggers a heat shock response readily detected in vivo using hsp70-GFP reporter zebrafish. Green fluorescent protein (GFP) is continuously expressed in the eyes, even in the absence of toxic stress.

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