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Allison M. Horst, Raja Vukanti, John H. Priester and Patricia A. Holden An Assessment of Fluorescence- and Absorbance-Based Assays to Study Metal-Oxide Nanoparticle ROS Production and Effects on Bacterial Membranes Small 9

Article first published online: 10 SEP 2012 | DOI: 10.1002/smll.201201455

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Five assays to study nanoparticle reactive oxygen species generation and effects on bacterial membrane integrity, membrane potential, and electron transport chain activity are assessed. A detailed quantitative assessment of nanoscale TiO2, CeO2, and ZnO interferences with assay reagents and function are included, in the context of a general scheme to test for nanoparticle interferences with fluorescence- and absorbance-based assays.

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